Packer L.'s Biothiols PDF

By Packer L.

This distinctive, state of the art reference offers a close account of the molecular mechanisms underlying the a number of features of biothiols-emphasizing the organic and medical implications of interactions among oxidants and biothiols.

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Chem. 259, 5606 (1984). 75 C. C. Winterbourn, Free Radical Biol. Med. 14, 85 (1993). [4] KINETICS OF THIOL REACTIONS 45 tion against oxidative damage has been reviewed. TM Evidence for the view that ascorbate may be about as important a radical sink as oxygen with respect to radical reaction cascades that involve thiyl radicals has been obtained from observations that the ascorbyl radical concentration in skin increases when carbon-centered radicals are induced by ultraviolet photolysis. 77 This could arise if the radicals are "repaired" by Eq.

Chem. 10, 725 (1972). 46 M. Z. Baker, R. Badiello, M. Tamba, M. Quintiliani, and G. Gorin, Int. J. Radiat. Biol. 41, 595 (1982). 38 TH1YLRADICALS [31 close to physiological pH. Similarly, detecting thiyl radicals through the observation of secondary chromophores illustrated in Eq. ( - 3 ) involves p H constraints, because the competing conjugation, Eq. (8b), has then to be avoided. Measurements utilizing Eq. ( - 3 ) are usually restricted to p H values <6. Thus both approaches often fail to cover the important physiological p H region, and extrapolations to p H - 7 are needed.

38 L. G. Forni, V. O. Mora-Arellano, J. E. Packer, and R. L. Willson, J. Chem. , Perkin Trans. 2, 1579 (1988). 39 B. C. Gilbert, in "Sulfur-Centered Reactive Intermediates in Chemistry and Biology" (C. -D. ), p. 135. Plenum, New York, 1990. 4o M. D. Sevilla, D. Becker, and M. Yan, Int. J. Radiat. Biol. 57, 65 (1990). ) have been subject to extensive investigation whereas ESR spectra of the thiyl radical itself are generally hardly observable or difficult to analyze. It should be noted, however, that ESR spectroscopy is used predominantly for identification rather than for kinetic experiments involving thiols.

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Biothiols by Packer L.

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